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Proteintech
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Proteintech
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Proteintech
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Rockland Immunochemicals
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EpiCypher
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Proteintech
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Image Search Results
Journal: Cell Death & Disease
Article Title: Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis
doi: 10.1038/s41419-021-04172-y
Figure Lengend Snippet: A β-Catenin (a marker of adherens junctions, green) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. B ZO2 (a marker of tight junctions, green) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. C TEX14 (a marker of a marker of testicular intercellular junctions, green) and β-actin (red) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. D Vimentin (a marker for Sertoli apical extensions, green) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. Nuclei are stained with DAPI.
Article Snippet: PPP6C antibody (rabbit, A300-844A; Bethyl Laboratories, Inc.); SYCP3 antibody (rabbit, NB300-231; Novus Biologicals); α-tubulin antibody (rabbit, 2144; Cell Signaling Technology, Inc.); β-actin antibody (mouse, 3700; Cell Signaling Technology, Inc.); Phospho-β-Catenin (Ser552) (D8E11) antibody (rabbit, 5651; Cell Signaling Technology, Inc.); SYCP3 antibody (mouse, sc-74569; Santa Cruz); γH2AX antibody (rabbit, 9718; Cell Signaling Technology, Inc.); MVH antibody (mouse, ab27591; abcam); SYCP1 antibody (rabbit, ab15090; abcam); SOX9 antibody (rabbit, AB5535, Sigma-Aldrich); PLZF antibody (goat, AF2944, R&D Systems); β-catenin antibody (rabbit, 51067-1-AP, Proteintech); ZO2 antibody (rabbit, 18900-1-AP, Proteintech);
Techniques: Marker, Immunofluorescence, Staining
Journal: International Journal of Molecular Sciences
Article Title: Dysfunction of Autophagy in Adipose Tissue Macrophages Regulated via FoxO1 in Obesity-Related Severe Acute Pancreatitis
doi: 10.3390/ijms26157206
Figure Lengend Snippet: Expression of autophagosome-localized SNARE proteins decreased in ATMs of obesity-related SAP. Western blot for the expression of STX17 and SNAP29 in adipose tissue ( A ) and relative protein expressions ( B ). ( C ) The mRNA levels of Stx17 and Snap29 in adipose tissue were examined via qPCR. Immunohistochemistry for STX17 ( D ) and SNAP29 ( E ) in adipose tissue (scale: 50 μm). Immunofluorescence images for F4/80 and STX17 ( F ) or SNAP29 ( G ) in adipose tissue (scale: 100 μm). Relative average fluorescence intensity of STX17 ( H ) and SNAP29 ( I ) in F4/80 + cells. Western blot for the expression of STX17 and SNAP29 in macrophages ( J ) and relative protein expressions ( K ). ( L ) The mRNA levels of Stx17 and Snap29 in macrophages were tested via qPCR. Immunofluorescence images for STX17 ( M ) and SNAP29 ( N ) in macrophages (scale: 100 μm). Relative average fluorescence intensity of STX17 ( O ) and SNAP29 ( P ) in macrophages. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
Article Snippet: Adipose tissue sections were incubated with an
Techniques: Expressing, Western Blot, Immunohistochemistry, Immunofluorescence, Fluorescence
Journal: International Journal of Molecular Sciences
Article Title: Dysfunction of Autophagy in Adipose Tissue Macrophages Regulated via FoxO1 in Obesity-Related Severe Acute Pancreatitis
doi: 10.3390/ijms26157206
Figure Lengend Snippet: FoxO1 regulated the expression of autophagosome-localized SNARE proteins in ATMs. Western blot for the expression of STX17 and SNAP29 in adipose tissue ( A ) and relative protein expressions ( B ) after the intervention of AS and LOM. Immunohistochemistry for STX17 ( C ) and SNAP29 ( D ) in adipose tissue (scale: 50 μm). Immunofluorescence images for F4/80 and STX17 ( E ) or SNAP29 ( F ) in adipose tissue (scale: 100 μm). Relative average fluorescence intensity of STX17 ( G ) and SNAP29 ( H ) in F4/80 + cells. Western blot for the expression of STX17 and SNAP29 in macrophages ( I ) and relative protein expressions ( J ). * p < 0.05, ** p < 0.01.
Article Snippet: Adipose tissue sections were incubated with an
Techniques: Expressing, Western Blot, Immunohistochemistry, Immunofluorescence, Fluorescence